Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.
Identifieur interne : 000421 ( Main/Exploration ); précédent : 000420; suivant : 000422Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.
Auteurs : Evangelia Vamvaka [Espagne] ; Richard M. Twyman [Royaume-Uni] ; Andre Melro Murad [Brésil] ; Stanislav Melnik [Autriche] ; Audrey Yi-Hui Teh [Royaume-Uni] ; Elsa Arcalis [Autriche] ; Friedrich Altmann [Autriche] ; Eva Stoger [Autriche] ; Elibio Rech [Brésil] ; Julian K C. Ma [Royaume-Uni] ; Paul Christou [Espagne] ; Teresa Capell [Espagne]Source :
- Plant biotechnology journal [ 1467-7652 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Analyse de profil d'expression de gènes (MeSH), Anticorps anti-VIH (biosynthèse), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (isolement et purification), Anticorps neutralisants (biosynthèse), Antigènes du VIH (immunologie), Endosperme (métabolisme), Endosperme (ultrastructure), Glycosylation (MeSH), Gènes de plante (MeSH), Oryza (génétique), Oryza (métabolisme), Protéomique (MeSH), Reproductibilité des résultats (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (génétique), Régulation positive (génétique), Transcriptome (génétique), Végétaux génétiquement modifiés (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- biosynthèse : Anticorps anti-VIH, Anticorps monoclonaux, Anticorps neutralisants.
- génétique : ARN messager, Oryza, Régulation négative, Régulation positive, Transcriptome.
- immunologie : Antigènes du VIH.
- isolement et purification : Anticorps monoclonaux.
- métabolisme : ARN messager, Endosperme, Oryza.
- ultrastructure : Analyse de profil d'expression de gènes, Endosperme, Glycosylation, Gènes de plante, Protéomique, Reproductibilité des résultats, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (isolation & purification), Antibodies, Neutralizing (biosynthesis), Broadly Neutralizing Antibodies (MeSH), Down-Regulation (genetics), Electrophoresis, Polyacrylamide Gel (MeSH), Endosperm (metabolism), Endosperm (ultrastructure), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Glycosylation (MeSH), HIV Antibodies (biosynthesis), HIV Antigens (immunology), Oryza (genetics), Oryza (metabolism), Plants, Genetically Modified (MeSH), Proteomics (MeSH), RNA, Messenger (genetics), RNA, Messenger (metabolism), Reproducibility of Results (MeSH), Transcriptome (genetics), Up-Regulation (genetics).
- MESH :
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Neutralizing, HIV Antibodies.
- chemical , genetics : RNA, Messenger.
- chemical , immunology : HIV Antigens.
- chemical , isolation & purification : Antibodies, Monoclonal.
- chemical , metabolism : RNA, Messenger.
- chemical : Broadly Neutralizing Antibodies.
- genetics : Down-Regulation, Oryza, Transcriptome, Up-Regulation.
- metabolism : Endosperm, Oryza.
- ultrastructure : Endosperm.
- Electrophoresis, Polyacrylamide Gel, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Glycosylation, Plants, Genetically Modified, Proteomics, Reproducibility of Results.
Abstract
Protein microbicides against HIV can help to prevent infection but they are required in large, repetitive doses. This makes current fermenter-based production systems prohibitively expensive. Plants are advantageous as production platforms because they offer a safe, economical and scalable alternative, and cereals such as rice are particularly attractive because they could allow pharmaceutical proteins to be produced economically and on a large scale in developing countries. Pharmaceutical proteins can also be stored as unprocessed seed, circumventing the need for a cold chain. Here, we report the development of transgenic rice plants expressing the HIV-neutralizing antibody 2G12 in the endosperm. Surprisingly for an antibody expressed in plants, the heavy chain was predominantly aglycosylated. Nevertheless, the heavy and light chains assembled into functional antibodies with more potent HIV-neutralizing activity than other plant-derived forms of 2G12 bearing typical high-mannose or plant complex-type glycans. Immunolocalization experiments showed that the assembled antibody accumulated predominantly in protein storage vacuoles but also induced the formation of novel, spherical storage compartments surrounded by ribosomes indicating that they originated from the endoplasmic reticulum. The comparison of wild-type and transgenic plants at the transcriptomic and proteomic levels indicated that endogenous genes related to starch biosynthesis were down-regulated in the endosperm of the transgenic plants, whereas genes encoding prolamin and glutaredoxin-C8 were up-regulated. Our data provide insight into factors that affect the functional efficacy of neutralizing antibodies in plants and the impact of recombinant proteins on endogenous gene expression.
DOI: 10.1111/pbi.12360
PubMed: 25845722
Affiliations:
- Autriche, Brésil, Espagne, Royaume-Uni
- Angleterre, Catalogne, Grand Londres, Vienne (Autriche)
- Barcelone, Londres, Vienne (Autriche)
- Université de Londres
Links toward previous steps (curation, corpus...)
Le document en format XML
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Twyman</name><affiliation wicri:level="1"><nlm:affiliation>TRM Ltd, York, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>TRM Ltd, York</wicri:regionArea><wicri:noRegion>York</wicri:noRegion></affiliation></author><author><name sortKey="Murad, Andre Melro" sort="Murad, Andre Melro" uniqKey="Murad A" first="Andre Melro" last="Murad">Andre Melro Murad</name><affiliation wicri:level="1"><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal</wicri:regionArea><wicri:noRegion>Distrito Federal</wicri:noRegion></affiliation></author><author><name sortKey="Melnik, Stanislav" sort="Melnik, Stanislav" uniqKey="Melnik S" first="Stanislav" last="Melnik">Stanislav Melnik</name><affiliation wicri:level="3"><nlm:affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Teh, Audrey Yi Hui" sort="Teh, Audrey Yi Hui" uniqKey="Teh A" first="Audrey Yi-Hui" last="Teh">Audrey Yi-Hui Teh</name><affiliation wicri:level="4"><nlm:affiliation>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">Université de Londres</orgName></affiliation></author><author><name sortKey="Arcalis, Elsa" sort="Arcalis, Elsa" uniqKey="Arcalis E" first="Elsa" last="Arcalis">Elsa Arcalis</name><affiliation wicri:level="3"><nlm:affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Altmann, Friedrich" sort="Altmann, Friedrich" uniqKey="Altmann F" first="Friedrich" last="Altmann">Friedrich Altmann</name><affiliation wicri:level="3"><nlm:affiliation>Department of Chemistry, Muthgasse, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Chemistry, Muthgasse, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Stoger, Eva" sort="Stoger, Eva" uniqKey="Stoger E" first="Eva" last="Stoger">Eva Stoger</name><affiliation wicri:level="3"><nlm:affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Rech, Elibio" sort="Rech, Elibio" uniqKey="Rech E" first="Elibio" last="Rech">Elibio Rech</name><affiliation wicri:level="1"><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal</wicri:regionArea><wicri:noRegion>Distrito Federal</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Julian K C" sort="Ma, Julian K C" uniqKey="Ma J" first="Julian K C" last="Ma">Julian K C. Ma</name><affiliation wicri:level="4"><nlm:affiliation>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">Université de Londres</orgName></affiliation></author><author><name sortKey="Christou, Paul" sort="Christou, Paul" uniqKey="Christou P" first="Paul" last="Christou">Paul Christou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida</wicri:regionArea><wicri:noRegion>Lleida</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Catalan Institution for Research and Advanced Studies (ICREA), Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Capell, Teresa" sort="Capell, Teresa" uniqKey="Capell T" first="Teresa" last="Capell">Teresa Capell</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida</wicri:regionArea><wicri:noRegion>Lleida</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (isolation & purification)</term><term>Antibodies, Neutralizing (biosynthesis)</term><term>Broadly Neutralizing Antibodies (MeSH)</term><term>Down-Regulation (genetics)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Endosperm (metabolism)</term><term>Endosperm (ultrastructure)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Glycosylation (MeSH)</term><term>HIV Antibodies (biosynthesis)</term><term>HIV Antigens (immunology)</term><term>Oryza (genetics)</term><term>Oryza (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Proteomics (MeSH)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Reproducibility of Results (MeSH)</term><term>Transcriptome (genetics)</term><term>Up-Regulation (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Anticorps anti-VIH (biosynthèse)</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (isolement et purification)</term><term>Anticorps neutralisants (biosynthèse)</term><term>Antigènes du VIH (immunologie)</term><term>Endosperme (métabolisme)</term><term>Endosperme (ultrastructure)</term><term>Glycosylation (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Oryza (génétique)</term><term>Oryza (métabolisme)</term><term>Protéomique (MeSH)</term><term>Reproductibilité des résultats (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (génétique)</term><term>Régulation positive (génétique)</term><term>Transcriptome (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>HIV Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>HIV Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Broadly Neutralizing Antibodies</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps anti-VIH</term><term>Anticorps monoclonaux</term><term>Anticorps neutralisants</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Down-Regulation</term><term>Oryza</term><term>Transcriptome</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Oryza</term><term>Régulation négative</term><term>Régulation positive</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes du VIH</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endosperm</term><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Endosperme</term><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Endosperm</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Glycosylation</term><term>Plants, Genetically Modified</term><term>Proteomics</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Endosperme</term><term>Glycosylation</term><term>Gènes de plante</term><term>Protéomique</term><term>Reproductibilité des résultats</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protein microbicides against HIV can help to prevent infection but they are required in large, repetitive doses. This makes current fermenter-based production systems prohibitively expensive. Plants are advantageous as production platforms because they offer a safe, economical and scalable alternative, and cereals such as rice are particularly attractive because they could allow pharmaceutical proteins to be produced economically and on a large scale in developing countries. Pharmaceutical proteins can also be stored as unprocessed seed, circumventing the need for a cold chain. Here, we report the development of transgenic rice plants expressing the HIV-neutralizing antibody 2G12 in the endosperm. Surprisingly for an antibody expressed in plants, the heavy chain was predominantly aglycosylated. Nevertheless, the heavy and light chains assembled into functional antibodies with more potent HIV-neutralizing activity than other plant-derived forms of 2G12 bearing typical high-mannose or plant complex-type glycans. Immunolocalization experiments showed that the assembled antibody accumulated predominantly in protein storage vacuoles but also induced the formation of novel, spherical storage compartments surrounded by ribosomes indicating that they originated from the endoplasmic reticulum. The comparison of wild-type and transgenic plants at the transcriptomic and proteomic levels indicated that endogenous genes related to starch biosynthesis were down-regulated in the endosperm of the transgenic plants, whereas genes encoding prolamin and glutaredoxin-C8 were up-regulated. Our data provide insight into factors that affect the functional efficacy of neutralizing antibodies in plants and the impact of recombinant proteins on endogenous gene expression. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25845722</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.</ArticleTitle><Pagination><MedlinePgn>97-108</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12360</ELocationID><Abstract><AbstractText>Protein microbicides against HIV can help to prevent infection but they are required in large, repetitive doses. This makes current fermenter-based production systems prohibitively expensive. Plants are advantageous as production platforms because they offer a safe, economical and scalable alternative, and cereals such as rice are particularly attractive because they could allow pharmaceutical proteins to be produced economically and on a large scale in developing countries. Pharmaceutical proteins can also be stored as unprocessed seed, circumventing the need for a cold chain. Here, we report the development of transgenic rice plants expressing the HIV-neutralizing antibody 2G12 in the endosperm. Surprisingly for an antibody expressed in plants, the heavy chain was predominantly aglycosylated. Nevertheless, the heavy and light chains assembled into functional antibodies with more potent HIV-neutralizing activity than other plant-derived forms of 2G12 bearing typical high-mannose or plant complex-type glycans. Immunolocalization experiments showed that the assembled antibody accumulated predominantly in protein storage vacuoles but also induced the formation of novel, spherical storage compartments surrounded by ribosomes indicating that they originated from the endoplasmic reticulum. The comparison of wild-type and transgenic plants at the transcriptomic and proteomic levels indicated that endogenous genes related to starch biosynthesis were down-regulated in the endosperm of the transgenic plants, whereas genes encoding prolamin and glutaredoxin-C8 were up-regulated. Our data provide insight into factors that affect the functional efficacy of neutralizing antibodies in plants and the impact of recombinant proteins on endogenous gene expression. </AbstractText><CopyrightInformation>© 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vamvaka</LastName><ForeName>Evangelia</ForeName><Initials>E</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-9818-4404</Identifier><AffiliationInfo><Affiliation>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Twyman</LastName><ForeName>Richard M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>TRM Ltd, York, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murad</LastName><ForeName>Andre Melro</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Melnik</LastName><ForeName>Stanislav</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Teh</LastName><ForeName>Audrey Yi-Hui</ForeName><Initials>AY</Initials><AffiliationInfo><Affiliation>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arcalis</LastName><ForeName>Elsa</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Altmann</LastName><ForeName>Friedrich</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Muthgasse, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stoger</LastName><ForeName>Eva</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department for Applied Genetics and Cell Biology, Molecular Plant Physiology and Crop Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rech</LastName><ForeName>Elibio</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Synthetic Biology, Parque Estacao Biologica, Brasilia, Distrito Federal, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Julian K C</ForeName><Initials>JK</Initials><AffiliationInfo><Affiliation>Molecular Immunology Unit, Division of Clinical Sciences, St. George's University of London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Christou</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Capell</LastName><ForeName>Teresa</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Plant Production and Forestry Science, School of Agrifood and Forestry Science and Engineering (ETSEA), University of Lleida-Agrotecnio Center, Lleida, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AY027531</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C529157">2G12 monoclonal antibody</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000080908">Broadly Neutralizing Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015483">HIV Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015488">HIV Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000080908" MajorTopicYN="N">Broadly Neutralizing Antibodies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056625" MajorTopicYN="N">Endosperm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015483" MajorTopicYN="N">HIV Antibodies</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015488" MajorTopicYN="N">HIV Antigens</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015854" MajorTopicYN="N">Up-Regulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2G12</Keyword><Keyword MajorTopicYN="N">HIV</Keyword><Keyword MajorTopicYN="N">endosperm</Keyword><Keyword MajorTopicYN="N">glycosylation</Keyword><Keyword MajorTopicYN="N">microbicides</Keyword><Keyword MajorTopicYN="N">rice</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>02</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>02</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25845722</ArticleId><ArticleId IdType="doi">10.1111/pbi.12360</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li><li>Brésil</li><li>Espagne</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Catalogne</li><li>Grand Londres</li><li>Vienne (Autriche)</li></region><settlement><li>Barcelone</li><li>Londres</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université de Londres</li></orgName></list><tree><country name="Espagne"><noRegion><name sortKey="Vamvaka, Evangelia" sort="Vamvaka, Evangelia" uniqKey="Vamvaka E" first="Evangelia" last="Vamvaka">Evangelia Vamvaka</name></noRegion><name sortKey="Capell, Teresa" sort="Capell, Teresa" uniqKey="Capell T" first="Teresa" last="Capell">Teresa Capell</name><name sortKey="Christou, Paul" sort="Christou, Paul" uniqKey="Christou P" first="Paul" last="Christou">Paul Christou</name><name sortKey="Christou, Paul" sort="Christou, Paul" uniqKey="Christou P" first="Paul" last="Christou">Paul Christou</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Twyman, Richard M" sort="Twyman, Richard M" uniqKey="Twyman R" first="Richard M" last="Twyman">Richard M. Twyman</name></noRegion><name sortKey="Ma, Julian K C" sort="Ma, Julian K C" uniqKey="Ma J" first="Julian K C" last="Ma">Julian K C. Ma</name><name sortKey="Teh, Audrey Yi Hui" sort="Teh, Audrey Yi Hui" uniqKey="Teh A" first="Audrey Yi-Hui" last="Teh">Audrey Yi-Hui Teh</name></country><country name="Brésil"><noRegion><name sortKey="Murad, Andre Melro" sort="Murad, Andre Melro" uniqKey="Murad A" first="Andre Melro" last="Murad">Andre Melro Murad</name></noRegion><name sortKey="Rech, Elibio" sort="Rech, Elibio" uniqKey="Rech E" first="Elibio" last="Rech">Elibio Rech</name></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Melnik, Stanislav" sort="Melnik, Stanislav" uniqKey="Melnik S" first="Stanislav" last="Melnik">Stanislav Melnik</name></region><name sortKey="Altmann, Friedrich" sort="Altmann, Friedrich" uniqKey="Altmann F" first="Friedrich" last="Altmann">Friedrich Altmann</name><name sortKey="Arcalis, Elsa" sort="Arcalis, Elsa" uniqKey="Arcalis E" first="Elsa" last="Arcalis">Elsa Arcalis</name><name sortKey="Stoger, Eva" sort="Stoger, Eva" uniqKey="Stoger E" first="Eva" last="Stoger">Eva Stoger</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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